DOI: 10.36347/sajp

Pages: 180-186

The fermentation medium composition and fermentation design greatly affects both the growth and productivity of extracellular enzymes from microorganisms. The objective of the research is to improve the α-amylase production by Bacillus licheniformis ATCC 6346 by optimizing fermentation medium compositions in submerged fermentation. The fermentation medium contained (L-) 2.0 g soluble starch, 1.0 g (NH4)2SO4, 2.0 g peptone, 1.0 g NaCl, 0.005 g FeCl3, 0,005 g MgCl2.6H2O, 0.005 g CaCl2.2H2O, 1.0 g KH2PO4 and 2.5 g K2HPO4 α. Production of α-amylase in the fermentation medium was 20.1 UmL-1. Optimizing the concentration of the components to (L-) 4 g soluble starch, 5 g (NH4)2SO4, 7.5 g K2HPO4, 4.0 g KH2PO4, 6 g peptone, 0.01g CaCl2.6H2O, 0.01 g MgCl2.6H2O and 0.01 g FeCl3 have improved the α-amylase production from 20.1 to 44.1 UmL-1