DOI: 10.36347/sajp

Pages: 504-509

Different biological methods are gaining recognition for the production of silvernanoparticles (Ag-NPs) due to their multiple applications. One of the most important applications of Ag-NPs is their use as an anti-bacterial agent. The use of plants in thesynthesis of nanoparticles emerges as a cost effective and eco-friendly approach. In this study preparation of silver nanoparticles by using leave extract of Eichhornia crassipes has been investigated. Characterization of different properties of the prepared nanoparticles by techniques like, UV-VIS spectroscopy, Zetasizer, TEM, and FTIR was carried out. In-vitro antibacterial study of the prepared nanoparticles on different microorganisms like Staphylococcus aureus, Bacillus subtilis, Escherichia hermanii, and Pseudomonas aeruginoss was carried out. The results showed the formation of silver nanoparticles in the range of 18–30 nm and average of 21.71+/- 4.42 nm in size. The silver nanoparticles showed the antimicrobial activity against Gram positive and Gram negative bacteria. Eichhornia crassipes was found to display strong potential for the synthesis of silver nanoparticles that can be used as antimicrobial agents by rapid reduction of silver ions