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Scholars Academic Journal of Pharmacy | Volume-7 | Issue-09
Detection and Estimation of Phenolic Acid and Flavonods in Leaves of Cadaba indica Lam by High Performance Thin Layer Chromatography
Thirumalai V, Nirmala P, Venkatanarayanan R
Published: Sept. 30, 2018 | 142 123
DOI: 10.21276/sajp.2018.7.9.4
Pages: 417-424
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Abstract
Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases of mankind. The present study mainly aimed to estimate the content of major constituent flavonoid present in various extracts of leaves of Cadaba indica collected from Tamilnadu. HPTLC method was adopted for determining the content of the active constituent present in the various extracts using marker compound Quercetin, rutin and gallic acid. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF254 as stationary phase and mobile phase as toluene: ethyl acetate: formic acid: methanol (3:6:1.6:0.4). The developed chromatogram was scanned at 254 nm using Camag scanner III. The chromatogram obtained and the peak profile of the components collected by scanning could made up the fingerprint of the various extracts of Cadaba indica leaves compared with antioxidants markers of flavonoids and phenolic acids. HPTLC finger print analysis showed that the presence of rutin, quercetin and gallic acid which may responsible for the anti-oxidant activity mentioned. This method is appropriate to determine the percentage of flavonoids and phenolic acids in various extracts of leaves of Cadaba indica.