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Scholars Academic Journal of Pharmacy | Volume-8 | Issue-03
Anti-Urolithiatic and Invitro-Invivo Anti-Oxidant Effects of Methanolic Extract of Thunbergia laurifolia on Ethylene Glycol-Induced Kidney Calculi in Rats
Amit Nayak, Madhu Garg, Saurabh Jain, Mohammed Azaz Khan, Deepak Kumar Jain, Nushrat Khan
Published: March 30, 2019 | 127 123
DOI: 10.21276/sajp.2019.8.3.5
Pages: 94-104
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Abstract
Oxidative stress acts as a vital mediator in the pathophysiology of urolithiasis. Renal calculi formation is one of the most general urological disorders. Urinary stone disease is a common disease which affects 10-12% of the population in developed countries. The objective of the present study is to investigate the antiurolithiatic and antioxidant activity of methanolic extract of Thunbergia laurifolia flowers on ethylene glycol-induced urolithiasis in Wistar albino male rats. Twelve male rats weighing 152-204 were used for this study. Urolithiasis was produced in Wistar albino rats by adding 0.75% v/v ethylene glycol (EG) to drinking water for 28 days. The methanolic extract of Thunbergia laurifolia flowers (TBL) was assessed for its preventive action in urolithiasis. In preventive treatment, the TBL given from 1st day to 28th day, various renal functional and injury markers such as urine volume, calcium, phosphate, uric acid, magnesium, urea and oxalate were evaluated using urine, serum and kidney homogenate. Antioxidant parameters such as DPPH radical scavenging activity, reducing power assay, lipid peroxidation, superoxide dismutase and Glutathione were also determined. The TBL treatment increased the urine output significantly compared to the control. The TBL treatment significantly reduced the urinary excretion of the calcium, phosphate, uric acid, magnesium, urea and oxalate and increased the excretion of citrate compared to EG control. The increased deposition of stone forming constituents in the kidneys of calculogenic rats were significantly lowered by treatment with TBL. It was also observed that alcoholic extract of TBL produced significant (P < 0.001) increased in lipid peroxidation (LPO), and decreased GSH, SOD.