DOI: https://doi.org/10.36347/sasjm.2025.v11i08.001

Pages: 744-747

Pus is a major sample received to the microbiology laboratory. A diverse group of pathogens will cause wound infections and the antibiotic sensitivity and resistant pathogens will be different in different hospitals. This may increase the antimicrobial resistance creating a great hazard globally. Aims and Objectives: To isolate and identify the bacteria from pus samples and perform their antibiotic susceptibility testing of the isolates. Materials and Methods: A total of 621 samples were collected from different departments irrespective of age and gender and processed in the department of Microbiology Guntur medical college, Guntur according to standard guidelines. Antimicrobial susceptibility testing was done by Kirby Bauer Disc Diffusion Testing and further ESBL detection was done by Disc Synergy test and results were interpreted. Results: More number of males participated in the present study with maximum age group being 45 -60 years. More number of samples were from Surgery. The pathogens isolated in the present study were Klebsiella, Escherichia coli, Pseudomonas, and Staphylococcus. The most antibiotics susceptible were Piperacillin and Tazobactum, Imipenem and Gentamycin. ESBL production in the present study was 44.5%. Conclusion: The present study revealed the pathogens causing pyogenic infections and their role in emergence of antimicrobial resistance which is becoming a global pandemic.