DOI: https://doi.org/10.36347/sajp.2025.v14i06.003

Pages: 120-127

Natural biopharmaceutics of plant origin are of great importance for human health and their application is increased in differemt areas. Important plants with natural biopharmaceutics are: Galanthus woronowi Losinsk, Galanthus nivalis L., Leucojum aestivum L., Lycoris aurea L., Lycoris radiata L., Narcissus tazetta L., Lactuca serriola, Eruca sativa, Heliotropium Bacciferum, Solanum glaucophyllum, Daucos carrota, Populus tremuloides, Cimicifuga racemosa, Trifolium pratense subsp. baeticum (Boiss.) Vicioso, Trifolium pratense subsp. kotulae (Pawl.) Soják Trifolium pratense subsp. pretense. Galantamine is a natural alkaloid with an in important role in treatment of Alzheimer’s disease. Estradiol is used against osteoporosis. Herbal products with Cimicifugae racemosae are applied for treating menopausal symptoms and exert anticancer properties. Trifolium pratense is applied as expectorant, sedative, and anti-inflammatory agent. In accordance the multi-target therapy of Alzheimer’s disease, the most important physical instrumental methods developed for the simultaneous determination of Galantamine hydrobromide and Pymadine in the model mixtures are HPLC method and TLC-densitometric method. The obtained from HPLC method data for limit of detection are 1.08.10-4 g/ml; for Galantamine hydrobromide and 1.32.10-4 g/m for Pymadine and are lower in comparison with the obtained by TLC-densitometric method data for LOD as 1.87.10-3 g/ml for Galantamine hydrobromide and 2.5.10-3 g/ml for Pymadine. For the determination of Galantamine hydrobromide and Pymadine by HPLC and TLC and for assay if Estradiol valerate by TLC was applied method of calibration curve.