DOI: 10.36347/sajp.2021.v10i12.006

Pages: 240-243

Rutin, a natural plant pigment and the most abundant plant flavonoid, work as a scavenger of various oxidizing species. In the present study an attempt has been made to develop a simple, precise, rapid, selective and cost-effective high-performance thin-layer chromatographic (HPTLC) method for estimation of rutin from polyherbal transdermal patch prepared for the treatment of arthritis. The method employed TLC aluminium plates precoated with silica patch 60F254 as the stationary phase and solvent system consisted of ethyl acetate, formic acid, glacial acetic acid. Densitometric analysis was carried out in the absorbance mode at 275nm. This system was found to give compact spots for rutin at Rf value of 0.54. Response was a linear function of the amount applied to the plate in the ranges 1-6 µg. The % of rutin from transdermal patch was found to be 100.94%, which was well within the limit. The developed HPTLC method would be an important tool in the quality control method for polyherbal formulations.